LipidSig 2.0: Integrating Lipid Characteristic Insights into Advanced Lipidomics Data Analysis

Enrichment analysis on our platform provides two main approaches: ‘Over Representation Analysis (ORA)’ and ‘Lipid Set Enrichment Analysis (LSEA)’ . ORA analysis illustrates significant lipid species enriched in the categories of lipid class. LSEA analysis is a computational method determining whether an a priori-defined set of lipids shows statistically significant, concordant differences between two biological states (e.g., phenotypes).

Demo dataset source (two-group): Adipose tissue ATGL modifies the cardiac lipidome in pressure-overload-induced left Ventricular failure (PLoS Genet. 2018)
Demo dataset source (multi-group): Lipidomic and biophysical homeostasis of mammalian membranes counteracts dietary lipid perturbations to maintain cellular fitness (Nat Commun 11, 1339. 2020)

LipidSig 2.0: Integrating Lipid Characteristic Insights into Advanced Lipidomics Data Analysis

Enrichment

Data Source

Data source

Uploaded data

Result

Over Representation Analysis

Configure your conditions by the control panel below, then click the 'Submit' button.

For input data preparation, please refer to FAQ. You can download inputs directly from the Differential expression analysis to ensure format accuracy.

The bar plot shows the top 10 significant up-regulated and down-regulated terms for datasets involving two groups and the top 20 for multiple groups.

Hover the mouse on a specific bar to view the corresponding detailed information.

For figure manipulation, please refer to FAQ.

The bar plot classifies significant lipid species into 'up-regulated' or 'down-regulated' categories based on their log2 fold change, according to a selected characteristic. Red bars indicate up-regulated, blue bars represent down-regulated, and grey bars signify non-significant.

Select a characteristic of interest from the drop-down list above the plot.

Hover the mouse on a specific bar to view the corresponding detailed information.

For figure manipulation, please refer to FAQ.

Lipid set enrichment analysis

Configure your conditions by the control panel below, then click the 'Submit' button.

For input data preparation, please refer to FAQ. You can download inputs directly from the Differential expression analysis to ensure format accuracy.

LipidSig 2.0: Integrating Lipid Characteristic Insights into Advanced Lipidomics Data Analysis

Enrichment

Data Source

Data source

Uploaded data

Result

Over Representation Analysis

Configure your conditions by the control panel below, then click the 'Submit' button. For input data preparation, please refer to FAQ. You can download inputs directly from the Differential expression analysis to ensure format accuracy.

The bar plot shows the top 10 significant up-regulated and down-regulated terms for datasets involving two groups and the top 20 for multiple groups.

Hover the mouse on a specific bar to view the corresponding detailed information. For figure manipulation, please refer to FAQ.

The bar plot classifies significant lipid species into 'up-regulated' or 'down-regulated' categories based on their log2 fold change, according to a selected characteristic. Red bars indicate up-regulated, blue bars represent down-regulated, and grey bars signify non-significant.

Select a characteristic of interest from the drop-down list above the plot. Hover the mouse on a specific bar to view the corresponding detailed information. For figure manipulation, please refer to FAQ.

Lipid set enrichment analysis

Configure your conditions by the control panel below, then click the 'Submit' button. For input data preparation, please refer to FAQ. You can download inputs directly from the Differential expression analysis to ensure format accuracy.

Configure your conditions by the control panel below, then click the 'Submit' button.

For input data preparation, please refer to FAQ. You can download inputs directly from the Differential expression analysis to ensure format accuracy.

Hover the mouse on a specific bar to view the corresponding detailed information.

For figure manipulation, please refer to FAQ.

Select a characteristic of interest from the drop-down list above the plot.

Hover the mouse on a specific bar to view the corresponding detailed information.

For figure manipulation, please refer to FAQ.

Configure your conditions by the control panel below, then click the 'Submit' button.

For input data preparation, please refer to FAQ. You can download inputs directly from the Differential expression analysis to ensure format accuracy.